About

I am currently working as a research fellow in the Department of Physiology at The University of Melbourne. My scientific interest lies in understanding the pathways that regulate skeletal muscle regeneration, with a specific focus on the endogenous population of skeletal muscle stem cells. I believe that a greater understanding of the regulation of skeletal muscle stem cells is crucial for the discovery of successful interventions that may have applications in regenerative medicine.

I completed my PhD under the guidance of Prof. Gordon Lynch in the Department of Physiology at The University of Melbourne in 2006. My doctoral research was focused on therapeutic treatment strategies for age-related muscle wasting and weakness.

I am a strong supporter of open-access for publicly funded research, and have recently come to appreciate the power of crowd-sourcing. Most of this blog will focus on skeletal muscle research (both unpublished and published findings), and provide detailed open-access protocols for all of the methods that I use for my research. I will also discuss my views on science policy in Australia, as I believe that this is a topic that really doesn’t receive enough attention in mainstream media.

My header image above shows skeletal muscle fibers (green) of a one day old mouse. Skeletal muscle stem cells, responsible for skeletal muscle regeneration are stained pink, while muscle nuclei are stained blue.

You can find out more about me on my about.me page, or follow me on Twitter or LinkedIn, or you can download a copy of my Curriculum Vitae

— This is my personal blog, it is not affiliated with The University of Melbourne, and all opinions in this blog are my own–

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2 thoughts on “About

  1. Yixuan

    Hi James! I’m a graduate student at UCSF, and am also interested in using satellite cells as a model to study stem cell metabolism. As such I was hoping you could offer some technical advice.

    I’ve been attempting to use magnetic beads / columns (Miltenyi MACS) but the yield is very poor so far. I was curious about how many satellite cells you usually get from sorting hindlimb muscle digests?

    For example according to your recent Cell Stem Cell paper (congratulations on that by the way), you seeded 50k SCs / well for the Seahorse assay; were you able to isolate that many SCs from a single mouse or did you have to pool muscles from several mice?

    Thanks!

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