Muscle methods

Here is a collection of methods that I use in the lab, written as lab methods rather than the (sometimes useless) “publication methods”. If there is something that I haven’t listed, then feel free to ask, I will try an populate this section on a monthly basis.

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4 thoughts on “Muscle methods

  1. Amrit

    Hi James
    I recently started research in myogenesis in cancer. And in quest of my search for answers to my questions I came across your interesting blog. I have a question for you.
    What does centrally localized nuclie in myofibre implies? Is it a new fibre in postinjury muscle or atrophy? I looked in literature but got confused. Thanks

    1. Sebastien Gingras

      While this process doesn’t seem to occur in humans muscle fibers, in mice fibers after they have been injured (such as in atrophied muscles), you often see nuclei migrating to the center of the fiber as opposed to their typical position in the periphery of muscle fibers. The reason for that phenomenon and why it occurs mostly in mice is not well understood. Hope this helps you a bit.

  2. HI, did you publish the “Effects of GFP on C2C12 metabolism” somewhere that I could cite? People in the field of reproduction use GFP overexpression as the standard to sort thier target cells and I need to convince them that this isn’t the way to go if I am doing XF analysis.
    Thanks!!

    1. Hi Dequina,
      No, we haven’t ever published this data, but I have been asked about it a lot. I guess the simple takeaway is to ensure you include both a GFP+ and GFP- control. This will allow you to determine if your observed difference is simply due to GFP.

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